Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 5th International Conference on Microbial Physiology and Genomics London, UK.

Day 1 :

OMICS International Microbial Physiology 2016 International Conference Keynote Speaker Mohannad Al Saghir photo
Biography:

Mohannad Al-Saghir has joined Ohio University in 2006 and holds a PhD in Biology with Microbiology and Plant Biology focus. His research is centered on microbial
evolution, medical microbiology and plant systematics and evolution. He is an Associate Professor of Biological Sciences at Ohio University Zanesville, USA. His
most recent publication is “Immunohistochemical Localization of Aspergillus and p53 in human lung tissues” published in American Journal of Molecular Biology
in 2015.

Abstract:

RADIUS (Risk Awareness of Disease and Infection Undergraduate Study) is a multipurpose project aimed at using modern
biotechnology to investigate the presence of parasitic and microbial contaminants in public parks, playgrounds and public
swimming pools in Zanesville, Ohio and provide Ohio University students with a superior learning experience through a
field-based, hands-on and problem-based curriculum centered on scientific and critical thinking. Launched in the Fall-2007,
RADIUS involved over 100 OUZ undergraduate students who collected swab and soil samples from public places and analyzed
them in the lab using both biochemical and molecular approaches. Many bacterial and fungal pathogens have been identified.
The project exposes students to modern biotechnology and its application to identify the potential environmental and
epidemiological risk factors and the dynamics of infectious agents in the local community. The RADIUS activities represent a
pilot model for incorporating biotechnology in teaching and learning, foster student-faculty research while providing service
to the community. Initial feedback and outcomes indicated the value of this form of improved undergraduate learning and
community service.

Keynote Forum

Ruslan Afasizhev

Boston University School of Dental Medicine, USA

Keynote: Uridylation-induced RNA degradation in mitochondria of trypanosomes: mechanisms, targets and evolution

Time : 11:05-11:35

OMICS International Microbial Physiology 2016 International Conference Keynote Speaker Ruslan Afasizhev photo
Biography:

Ruslan Afasizhev has completed his PhD from Institute of Molecular Biology, Russian Academy of Sciences. He is a Professor of Molecular and Cell Biology at
Boston University Medical Campus, USA. He has published more than 50 papers in high ranking journals.

Abstract:

Massive U-insertion/deletion mRNA editing in mitochondria of trypanosomes inspired earlier efforts to discover enzymes
responsible for RNA uridylation. Studies of RNA editing ultimately led to identification of two terminal uridyltransferases
(TUTases): RNA editing TUTase 1 (RET1) and RNA editing TUTase 2 (RET2). Subsequent work demonstrated that RET2
functions as subunit of the RNA editing core complex and is responsible for internal mRNA editing. Conversely, RET1 was
implicated in 3' modification of mRNA, rRNA and guide RNAs. Recently, we identified a protein complex composed of RET1
TUTase, DSS1 3'-5' exonuclease and three additional subunits. This complex, termed mitochondrial 3' processome (MPsome),
is responsible for primary uridylation of ~800-nt gRNA precursors, their processive degradation to a mature size of 40-60
nucleotides and secondary U-tail addition. Both strands of the gRNA gene are transcribed into sense and antisense precursors
of similar lengths. Head-to-head hybridization of these transcripts blocks symmetrical 3'-5' degradation at a fixed distance
from the double-stranded region. Together, our findings suggest a model in which gRNA is derived from the 5' extremity of
a primary molecule by uridylation-induced, antisense transcription-controlled 3'-5' exonucleolytic degradation. Remarkably,
we also established that MPsome-catalyzed 3'-5' degradation also represents the major pathway for mRNA processing and
degradation. These finding poses a logistical challenge to the established paradigm of multicistronic mitochondrial transcription
and raises the questions of mRNA 5' end modification and 3' end definition. We will discuss potential existence of gene specific
promoters and the role of MERS1 NUDIX hydrolase in mRNA 5' processing and stabilization. We will also present data
demonstrating that, in contact to antisense RNA-based 3' end definition of gRNAs, the mature 3' ends of pre-mRNAs are
generated by protein-based mechanism that blocks the MPsome and stimulates mRNA polyadenylation.